FastQCFastQC Report
Fri 4 Nov 2016
6_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5988
Sequences flagged as poor quality0
Sequence length1-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTTCCGATC150.250501002004008Illumina Single End Adapter 1 (100% over 12bp)
GTGGTTTCTGGCGGAGGTAAACGGATTTGCGTGCCAGTCGTCTCTGCTTT90.15030060120240482No Hit
GTCCAGGTAGCCAAAAAATCCCGAGCAAAACAACATTGTGAATGAAGCAA80.13360053440213762No Hit
CTCGTACACCTCATACGCATTCAGCTGACAAATAAAGGCAAATGTGAAAACAGAAAGGCC70.11690046760187041No Hit
CAACAAACCACAGCCGATCTATTCCTTGCTAAATATACGCCGGGTGCATT70.11690046760187041No Hit
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT60.1002004008016032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGC100.0094005205130.65347100
TCACCCT100.00997368128.116595
GCCCCAC200.001068185183.168071
Produced by FastQC (version 0.11.5)