FastQCFastQC Report
Fri 4 Nov 2016
6_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15346013
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT195880.12764227425064736No Hit
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT170880.11135139791684003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT269200.059.8789631
ACAGTTT402350.040.509692
CAGTTTC468750.034.7474823
GTTTCTG946950.033.5869981
TTCTGTA1012350.031.2188383
TCTGTAC1022950.030.8960464
TGTACTA1067850.029.5605966
ACATAAG70950.029.5030216
TTACCAG82850.029.2414763
TTGATAC111650.029.2077543
CTGTACT1084100.029.1395035
TACTATA1086700.029.0183688
TGATACA111750.028.8718324
CATAAGG71350.028.771737
GTACTAT1102400.028.6512817
GATAAAC74650.028.42451
TTTCTGT1122500.028.350452
ATACAGT111950.028.3052376
ACTATAT1134650.027.8035249
GATACAG115500.027.4358775
Produced by FastQC (version 0.11.5)