FastQCFastQC Report
Fri 4 Nov 2016
6_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10177
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT170.16704333300579738No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA160.15721725459369165No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG160.15721725459369165No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG160.15721725459369165No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG130.12773901935737447No Hit
ATCTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATG120.11791294094526873No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA120.11791294094526873No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG120.11791294094526873No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA120.11791294094526873No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA110.10808686253316302No Hit
ATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGT110.10808686253316302No Hit
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCCGCGAAATCTCGTA110.10808686253316302No Hit
CAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAG110.10808686253316302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCG100.009651348130.06215115
GAGTGCC100.00981616129.33147114
GATCGGA401.8189894E-12113.403951
AGCCAAA209.782175E-485.4740120
GTAGGGA209.782175E-485.4740118
ATCGGAA402.3985194E-885.052962
TCGGAAG351.1097691E-681.002823
GGGAGAG300.004884627656.98267721
CGGAAGA350.00916585148.6016924
Produced by FastQC (version 0.11.5)