FastQCFastQC Report
Fri 4 Nov 2016
7_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14926437
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC437650.2932046006692689No Hit
CCCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCG235540.1578005521344444No Hit
CTTCGCCAAATATGAGACCACACGAAGAAGAAGGAGCGCTTTGTCCCTCC192890.12922708882233583No Hit
CTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTGCT186530.12496619253476232No Hit
CCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCGC185020.12395456464258685No Hit
GTCAATTTCTTTAAGTTTCACTCTTGCGAACGTACTCCCCCCTGAGACTG182740.12242707352062651No Hit
CCCCGAACTACCCTCCTTCATTCCTGGATGCCGTGAGTTGAGGGAAGGCA177830.11913760799044004No Hit
CTCGCAATTTCCAGCTAAGTAGGGTAAAACCAACCTGTCTCACGACGGTC168740.11304774206999299No Hit
ACCGCACGCTCCACGTCTTGTGGTGCCATTCCGTCAATTTCTTTAAGTTT166920.11182842898141063No Hit
CTCTAATCATCTTCGATCCCCACACTTTGGTTCTTGATTGAGGAAGGTAT156380.10476713230357652No Hit
CGTAAATTTAATATATTCATTGGCGAGACGAGGAACGGGATGAAAAATAA151180.10128338062191265No Hit
CCCTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTG149280.10001047135361238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT40150.053.544365113
CTAGGTT40800.053.191456114
TAGGTTA44000.049.496357115
AGGTTAG47200.046.014877116
CGCTAGG47350.045.253628112
GCGCTAG47500.044.831787111
GGTTAGG50700.042.85334117
AGCTAAG49900.042.70376213
CTAAGTA54100.040.03339415
TAGGGTA55100.038.99735620
GGCGCTA55800.038.046696110
CCCTTAG59400.036.40162793
TAGGAAG61300.036.05813120
TTAGGAA62150.035.540485119
CCCACAT172450.035.4959221
TAAGTAG62200.034.44698316
AGTAGGG62100.034.41056418
TTAGCGT63350.034.1145296
CAGCTAA64100.033.6962212
CCCAGGT163000.033.59480331
Produced by FastQC (version 0.11.5)