FastQCFastQC Report
Fri 4 Nov 2016
7_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5747
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAAAGAAAAGAGAGAGAGAGCGCCCCTCACGCTGCAATGCCTCGTGC80.1392030624673743No Hit
GTGGTTTCTGGCGGAGGTAAACGGATTTGCGTGCCAGTCGTCTCTGCTTT80.1392030624673743No Hit
CTGCGCAGGATGTCCACTAATTCCGCCTGTGTCTGCAGGGCACCCAACTG60.10440229685053071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCAT100.009089959132.03192105
TGCCTTC100.009994763127.9484591
ATGCCTT100.009994763127.9484590
Produced by FastQC (version 0.11.5)