FastQCFastQC Report
Fri 4 Nov 2016
7_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14926437
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT404540.2710224817885206No Hit
GTCAAACAGACGCGCAAGGGAGAAGATATCGTTGCCAGTACTTCTCTCGT210990.14135322448351204No Hit
CAGGACTACCCGCCGAACTTAAGCATATTACTAAGCGGAGGAAAAGAAAA176600.11831356672727725No Hit
GGCAGGACTACCCGCCGAACTTAAGCATATTACTAAGCGGAGGAAAAGAA158950.10648890957701425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT228500.061.638391
TTACCAG112100.046.060093
ACAGTTT345100.041.2333222
CCAGATC136250.037.5159156
CTTTACC145950.036.2924651
TACCAGA142300.036.2447784
ACCAGAT143850.035.935595
TCCGGAC143550.035.4120711
CAGATCC144550.035.282587
CAGTTTC407300.034.7654573
GTTTCTG821150.033.554281
GTCAAAC87300.032.1335451
GATCCGG160150.031.8123599
ACATAAG61250.031.7693316
TTCTGTA881450.031.0613653
ATCCGGA163150.030.9431610
TCTGTAC884350.030.901024
CATAAGG61900.030.592217
GATAAAC65900.030.0534761
ACAGGGT170450.029.8651216
Produced by FastQC (version 0.11.5)