FastQCFastQC Report
Fri 4 Nov 2016
7_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8734
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT150.1717426150675521No Hit
CTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACC150.1717426150675521No Hit
CAACTCCCTGCGCCATATCTCAATTATTGCTGTAATGTTCATCGTCTTCT140.16029310739638197No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA120.13739409205404168No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG120.13739409205404168No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG120.13739409205404168No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC110.12594458438287154No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA110.12594458438287154No Hit
ACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGAC100.11449507671170141No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA100.11449507671170141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTTC100.009512548130.57237104
TCCACTC100.009512548130.57237108
CTTTGAC100.009512548130.57237100
TCTTTGA100.009702067129.7189599
CTTAAAA152.8826244E-4116.0643355
ACTTAAA209.0494467E-487.0482454
TTAAAAA209.0494467E-487.0482456
AAAGAGT209.2634175E-486.5421524
GATCGGA209.702666E-485.547411
ATCGGAA250.002352655668.4379352
GAAAGAG300.004626594557.69476723
TTTCTGT300.00484519757.0316122
GTTTCTG300.00484519757.0316121
Produced by FastQC (version 0.11.5)