FastQCFastQC Report
Fri 4 Nov 2016
8_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14319982
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC302780.21143881326107813No Hit
CCCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCG262100.1830309563238278No Hit
CTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTGCT235490.16444853073139337No Hit
CCCCGAACTACCCTCCTTCATTCCTGGATGCCGTGAGTTGAGGGAAGGCA211670.14781443161031904No Hit
CCCTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTG208200.14539124420687122No Hit
GTCAATTTCTTTAAGTTTCACTCTTGCGAACGTACTCCCCCCTGAGACTG196660.1373325748593818No Hit
CTCTAATCATCTTCGATCCCCACACTTTGGTTCTTGATTGAGGAAGGTAT179270.12518870484613737No Hit
CCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCGC171390.11968590463312034No Hit
CGTAAATTTAATATATTCATTGGCGAGACGAGGAACGGGATGAAAAATAA166520.11628506236949179No Hit
ACCGCACGCTCCACGTCTTGTGGTGCCATTCCGTCAATTTCTTTAAGTTT162050.11316355006591489No Hit
CTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCG153130.10693449195676363No Hit
GTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCGGTC151520.10581018886755583No Hit
ACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCGGTCAAG147280.1028492912910086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT25650.056.236713113
CTAGGTT26050.055.986317114
TAGGTTA30400.048.27844115
AGGTTAG31600.046.532837116
GCGCTAG32250.045.241463111
CGCTAGG34450.042.019184112
GGTTAGG36850.040.163837117
AGCTAAG34750.039.131513
TGTTACG55500.036.4872783
CTAAGTA37950.036.13821415
GGCGCTA41750.034.902733110
ACGCCTA59350.034.8690768
CCCTTAG40200.033.8618593
TAGGGTA40700.033.84429620
TAAGGGG112800.033.14236571
TAAGTAG42400.031.93664216
TAGGAAG49700.031.540138120
TTAGGAA48050.031.522408119
CCCAGGT119550.031.49951431
CCAGGTT121050.031.29811332
Produced by FastQC (version 0.11.5)