FastQCFastQC Report
Fri 4 Nov 2016
8_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14319982
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT480790.33574762873305286No Hit
CTGCCAGTAGTCATATGCTTGTTTCAAGGACTTAGCCATGCATGCCTCAG170050.11875014926694741No Hit
ACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCTCGAT156950.10960209307525667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT236650.063.693921
TTACCAG119100.050.6437073
ACAGTTT333900.045.2470862
TACCAGA145400.041.643234
CCAGATC152000.039.4176376
CTTTACC155100.039.298871
ACCAGAT153600.039.1938325
TCCGGAC158900.037.749411
CAGATCC159100.037.696557
CAGTTTC416150.036.3740233
GTTTCTG849750.034.877251
GATCCGG176700.034.108149
ATCCGGA176900.034.0376810
TTCTGTA910050.032.299593
TCTGTAC917750.032.1426854
ACAGGGT187350.032.08718516
AGATCCG192050.031.2900078
TGTACTA973550.030.3312876
CTGTACT983150.029.9926475
CCGGACA201000.029.87263712
Produced by FastQC (version 0.11.5)