FastQCFastQC Report
Fri 4 Nov 2016
8_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7938
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG160.20156210632401111No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG140.1763668430335097No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA130.163769211388259No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA120.15117157974300832No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT120.15117157974300832No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA110.1385739480977576No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG100.12597631645250693No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA100.12597631645250693No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC90.11337868480725624No Hit
CTTTGACGGTGGGCATCTCTACTTGTCTAAGGGGCAGCAGGACATACCAG90.11337868480725624No Hit
ATTGTTATAAGGTGCTCTCGGCTAAAATATACTTGAAGGCTTTCATGGCC90.11337868480725624No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA80.10078105316200556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG100.008375291136.08594106
CATTAAC100.009188714131.9621394
TGGGGAA100.009188714131.9621395
TTAACTG100.009188714131.9621396
TCATTAA100.009188714131.9621393
ATATTTC100.009188714131.9621394
GCATGCT350.00826031449.7685763
Produced by FastQC (version 0.11.5)