FastQCFastQC Report
Tue 25 Oct 2016
1_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16153850
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCT31400.030.952108111
GACTAGG31600.030.402225109
TGACTAG35400.027.730406108
ACTAGGC35450.027.25484110
CAGCTAG42150.022.89954669
CTAGAAC43100.022.44686372
TAATCTA42450.021.704744119
CTAATCT46300.020.083448118
GTGACTA49100.020.011324107
CCAGCTA49600.019.2113768
CTATAAC48050.018.7866947
AGCTAGA51400.018.5618870
CAACCTA49350.018.32396999
CGCTAAC55400.016.805527117
ACCGCTA55400.016.731274115
AATCTAA71750.016.603106120
TAGGCTT58650.016.43815112
GCTAGAA59300.016.19912171
GTAAGTA60800.016.0292186
TAGCTAA21200.015.86327743
Produced by FastQC (version 0.11.5)