FastQCFastQC Report
Tue 25 Oct 2016
1_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7134
Sequences flagged as poor quality0
Sequence length2-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGCAAGTCTCTACCCAACCTCGCTCTGTATAGCGAAATTGAGCAA80.112139052425007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG100.00819582136.97435117
CACTGAA100.009069886132.4462797
TATCACT100.009069886132.44627101
ACAGACT100.009764568129.2419394
TGTTTCG100.009764568129.2419392
TTTGATG100.009764568129.2419393
Produced by FastQC (version 0.11.5)