FastQCFastQC Report
Tue 25 Oct 2016
1_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16153850
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT172340.10668664126508541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT312250.057.7519381
ACAGTTT430400.042.343122
CAGTTTC523650.034.6155933
GTTTCTG1061700.033.435771
TTCTGTA1116050.031.4768833
TCTGTAC1123150.031.3501634
TGTACTA1202100.029.253026
CTGTACT1221750.028.7641985
TTTCTGT1231450.028.7333932
TACTATA1233800.028.4999968
GTACTAT1251250.028.1152847
ACTATAT1282650.027.3934389
CTATATT1320850.026.63353510
AGTTTCT694600.026.2213524
TATATTG1415950.024.81687511
ATACAGT127800.021.9255776
GATACAG131100.021.2858145
ATATTGG532250.021.173912
TTGATAC135700.021.0754533
ATAGTAC32700.020.8915566
Produced by FastQC (version 0.11.5)