FastQCFastQC Report
Tue 25 Oct 2016
1_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9308
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGA100.008686424134.61935119
CATAACG100.008686424134.61935118
CGACACC100.00885611133.75641117
AACGAAT100.00885611133.75641115
AAACGAA100.009027983132.90446114
ACCCCAC100.009737624129.60248106
AGGCACC100.009737624129.60248107
TTGAAGC100.009737624129.60248103
TTTACGG100.009737624129.60248104
CACGGCA100.00992064128.80246102
TGTGTGT350.007147952451.69116670
TTCTGTA506.7717367E-444.873123
TCTGTAC506.7717367E-444.873124
TTTCTGT550.001082753840.7937432
GTACTAT600.001660883837.3942647
CTGTACT600.001660883837.3942645
TACTATA600.001660883837.3942648
ACTATAT600.001660883837.3942649
CTATATT650.00246046334.51778410
TATATTG650.00246046334.51778411
Produced by FastQC (version 0.11.5)