FastQCFastQC Report
Tue 25 Oct 2016
2_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18114330
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGG35650.029.40869109
CTAGGCT36450.029.147541111
ACTAGGC39450.026.923203110
TGACTAG42800.024.802929108
TAATCTA49800.022.409536119
CTAATCT51400.021.696764118
CAGCTAG45800.021.65531569
CTAGAAC46800.021.38484672
CAACCTA54100.020.13598399
GTGACTA55350.019.537252107
CTATAAC57550.018.7127557
AATCTAA79750.017.47893120
TAGGCTT64900.017.224655112
GTTCTAA66300.017.108156115
CCAGCTA59250.016.82264168
AGCTAGA60400.016.43725670
ACCTACC72700.015.503295101
TAGCTAA20550.015.44067943
AACCTAC73750.015.287483100
CTAGGTT9750.015.066461114
Produced by FastQC (version 0.11.5)