FastQCFastQC Report
Tue 25 Oct 2016
2_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11498
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAAT100.007993308138.56215117
GATTGGT100.008524042135.62962110
CGGTTTG100.008524042135.62962112
TTGATAC153.3588274E-4111.9388663
GATACAG200.001054134183.954155
ATTGATA200.001054134183.954152
ATATTGC200.001054134183.9541512
ATACAGT200.001054134183.954156
TGATACA250.002555582467.1633154
AGTTTCT250.002555582467.16331510
TACAGTT300.00526222855.9694337
CTATATT501.0049871E-555.9694310
AGGGAAA402.2983253E-455.9694320
TATATTG551.7671864E-550.881311
TGTACTA551.7671864E-550.88136
CTGTACT551.7671864E-550.88135
TACTATA551.7671864E-550.88138
ACTATAT551.7671864E-550.88139
TCTGTAC454.1116512E-449.7506034
TATTGAT350.00968082347.97381
Produced by FastQC (version 0.11.5)