FastQCFastQC Report
Tue 25 Oct 2016
3_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12912495
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCT22300.024.99375111
GACTAGG22800.024.42832109
CTAGGTT8850.024.286135114
GCTAGGT8900.022.44208113
TGACTAG25950.022.095678108
ACTAGGC26000.022.075975110
TAATCTA31350.021.748705119
AGACCTA22300.020.26018197
TAGGTTA10600.019.752726115
GACCTAA23050.019.42669998
CCTATGG32550.019.162267
CTAATCT35700.018.965252118
TAAGGGG33300.018.61630871
TCCTATG34700.018.46705266
TAGCTAA16350.018.04946343
CTAGAAC28550.018.0446372
CAACCTA36250.017.65590599
CAGCTAG29700.017.100369
GTGACTA34200.017.052286107
CTATAAC37400.016.8392227
Produced by FastQC (version 0.11.5)