FastQCFastQC Report
Tue 25 Oct 2016
3_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5669
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAAAGAGGTGCATTCCTTTTATCAGGATACATATAAATTAATTAAG150.2645969306756042No Hit
GCTTCATCACCATCGCCAGTGGGAACTGCGCCCCCGCAGAATCCACA80.14111836302698888No Hit
CTTGGAAACAAGTGGGAATAGAACCTGGAGACTCACACGTCCTTCTTTAT70.12347856764861528No Hit
GTGGGTGGGAAGGTAAATAAAAATAAGAGTTATCCAGCCGCCATCGCCTC60.10583877227024166No Hit
GCTCTTCCGATC60.10583877227024166Illumina Single End Adapter 1 (100% over 12bp)
GTGGGAAGGTAAATAAAAATAAGAGTTATCCAGCCGCCATCGCCTCCCTG60.10583877227024166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAGT100.008769466133.6421115
AGCAGTC100.008769466133.6421116
AGTCACG100.008769466133.6421119
GTAGCAG100.008769466133.6421114
TAAAATC100.009051283132.25110
ACGTAGC100.009051283132.25112
TCACGTA100.009051283132.25110
CGCGCCA100.0096328305129.5510397
Produced by FastQC (version 0.11.5)