FastQCFastQC Report
Tue 25 Oct 2016
3_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12912495
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT233950.060.3130651
ACAGTTT331150.043.1163752
CAGTTTC392100.036.090793
GTTTCTG788000.032.8565181
TTCTGTA836400.030.7212943
TCTGTAC836200.030.6882384
TGTACTA877850.029.2420886
CTGTACT896450.028.6332515
TACTATA899350.028.492818
GTACTAT913300.028.1331337
TTTCTGT932200.027.7680242
AGTTTCT517800.027.5425454
ACTATAT935050.027.4118869
CTATATT967850.026.5499610
TATATTG1041550.024.7501611
ATATTGG393050.021.70180912
ATACAGT89250.021.3124526
GATACAG94900.020.1639465
CCCCCCT94950.019.7271255
TTGATAC105050.019.3149173
Produced by FastQC (version 0.11.5)