FastQCFastQC Report
Tue 25 Oct 2016
3_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8600
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAC100.009600379130.16119
CACGCAA100.009794229129.298115
TCACGCA100.009794229129.298114
ATCACGC100.009794229129.298113
ATCCATC100.0099906605128.44737109
CATCCAT100.0099906605128.44737108
CATCACG100.0099906605128.44737112
GCATCCA100.0099906605128.44737107
GAGCGTC250.002342094468.5052649
AGAGCGT250.002342094468.5052648
Produced by FastQC (version 0.11.5)