FastQCFastQC Report
Tue 25 Oct 2016
4_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16500795
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGG28700.025.398611109
TAATCTA40550.024.861782119
CTAGGCT30150.024.238258111
CTAATCT45400.022.366482118
ACTAGGC34200.021.828598110
TGACTAG34550.020.977066108
GCTAGGT9650.019.686762113
CAACCTA48850.019.56998399
CTATAAC49750.019.232057
CTAGGTT10400.019.039568114
CAGCTAG36500.018.6228269
CCCCGAT51050.018.1782651
CTAGAAC38900.017.80943972
AATCTAA68650.017.567057120
TAGGTTA11000.017.490126115
CCTATGG36200.017.29720367
ACCGCTA52100.017.144833115
TCCTATG35650.017.06164266
GTTCTAA58500.017.030985115
CGCTAAC54900.015.96881117
Produced by FastQC (version 0.11.5)