FastQCFastQC Report
Tue 25 Oct 2016
4_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7198
Sequences flagged as poor quality0
Sequence length2-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAAAGAGGTGCATTCCTTTTATCAGGATACATATAAATTAATTAAG80.11114198388441232No Hit
CTCGACTAACATGAATCATTTCTCGATCCCATAGTTTTTCCTGTGAAATA80.11114198388441232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGA100.008293831136.44067116
ATCCTGT100.008507535135.29411108
TGTTGCA100.008507535135.29411112
TTGCATC100.008507535135.29411114
CTGAATC100.00894585133.05786104
GGCTGCA100.009398909130.894394
ACATCTG100.009398909130.8943100
GCTGCAC100.009398909130.894395
GACGACA100.009398909130.894393
GCACATC100.009398909130.894398
TGGCTGC100.009398909130.894393
Produced by FastQC (version 0.11.5)