FastQCFastQC Report
Tue 25 Oct 2016
4_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16500795
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT186930.11328545079191639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT318650.061.935321
ACAGTTT438150.045.573672
CAGTTTC527100.037.7077673
GTTTCTG1077100.033.9530071
TTCTGTA1120850.032.3237273
TCTGTAC1124100.032.2719544
TGTACTA1184750.030.5710956
TACTATA1207900.030.0114758
CTGTACT1210600.029.9391125
GTACTAT1226150.029.5304977
TTTCTGT1239250.029.464142
ACTATAT1252650.028.9417929
AGTTTCT699250.028.6072774
CTATATT1295150.028.04677210
TATATTG1390350.026.13573311
ATATTGG509950.022.38403112
ATACAGT122150.021.040436
GATACAG125450.020.9505585
ATATTGA535900.020.56377212
TTGATAC131850.019.9761683
Produced by FastQC (version 0.11.5)