FastQCFastQC Report
Tue 25 Oct 2016
4_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9320
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTGTACTATATTGCTGTTGCCAGAAGGAAATTTACAATGTGCTTGT100.1072961373390558No Hit
GTTTCTGTACTATATTGAAAGGTTGGTAGATAAGGGAAGTAGGCAGCAGT100.1072961373390558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATTT100.007901684138.88965117
ATTCGCA100.008404039136.07431103
TATTTGC100.008404039136.07431101
ATTTGCC100.008404039136.07431102
AGGAACG100.008927167133.3708596
AAGGAAC100.008927167133.3708595
CATATTT100.009287664131.6274492
CACATAT100.009657701129.9290390
GTACGGA100.009657701129.9290389
CCGGCGC207.581349E-490.98945674
GTATGTG250.001975755471.49940566
TGTACTA351.3131877E-462.543486
CTGTACT351.3131877E-462.543485
TTCTGTA351.3492748E-462.2054063
TCTGTAC351.3492748E-462.2054064
ACTATAT402.5414058E-454.725549
CTATATT454.5459482E-448.64492810
GTACTAT454.5459482E-448.6449287
TACTATA454.5459482E-448.6449288
TTTCTGT454.6704878E-448.381982
Produced by FastQC (version 0.11.5)