FastQCFastQC Report
Tue 25 Oct 2016
5_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11325488
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGG14100.021.362625109
ACCGCTA43550.021.016575115
CTAGGCT14650.020.858944111
TAAGGGG25000.020.72528371
CGCTAAC47400.019.75684117
TTAATCA58250.018.75933642
CTAGGTT8450.018.487242114
TGACTAG17000.018.397223108
ACTAGGC16750.018.110037110
TAGGTTA8700.018.081879115
GCTAACA54450.018.000456118
CCGCTAA55050.017.14739116
CTTAATC62550.017.0083441
GCTAGGT9250.016.760775113
AGACCTA14800.015.42021297
AGTAAGG34750.015.21353269
AATTAAC62150.015.030047102
GACCTAA15750.014.9769398
CTAAATC64350.014.60647277
CTAGAAC18350.013.974872
Produced by FastQC (version 0.11.5)