FastQCFastQC Report
Tue 25 Oct 2016
5_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5619
Sequences flagged as poor quality0
Sequence length1-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTTCCGATC270.4805125467164976Illumina Single End Adapter 1 (100% over 12bp)
GTAATTTTGAAGAAGAAGAAGACAAGGTTTTTGTGAAACTCAAAAGCTTT60.10678056593699946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTT100.0095557105129.8279694
CTTGCGG100.0095557105129.8279693
Produced by FastQC (version 0.11.5)