FastQCFastQC Report
Tue 25 Oct 2016
5_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11325488
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT125990.11124465453497456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT211450.045.54321
ACAGTTT297050.032.6713332
GTTTCTG655200.030.956941
TTCTGTA665650.030.1528973
TCTGTAC667850.029.9949324
CAGTTTC329150.029.555583
TGTACTA686050.029.0678836
TACTATA696850.028.6780228
CTGTACT702650.028.461195
GTACTAT710200.028.1379497
ACTATAT730150.027.3639189
TTTCTGT741850.027.3029652
CTATATT746650.026.85206810
TTGATAC100450.026.1994363
CCCCCCT100450.025.8583815
ATACAGT106150.025.1210426
TATATTG806300.024.82398611
TGATACA108450.024.3740484
GATACAG111250.023.9679435
CCCCCTT111750.023.14226
Produced by FastQC (version 0.11.5)