FastQCFastQC Report
Tue 25 Oct 2016
5_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16482
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTTCCGATC220.13347894672976582Illumina Single End Adapter 1 (100% over 12bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTG100.009799412129.67375117
TCGACGT206.0481616E-6116.83066645
GGGTAAG207.528117E-6111.8287421
TGTAGGG251.6719423E-7111.8287417
TAAGGAA153.3984415E-4111.8287419
GTGGTGT153.3984415E-4111.8287413
CGGTAGA153.482284E-4111.148944
GTGTAGG402.401066E-1097.8501516
GTAGGGA351.0875738E-895.853218
GATCGGA700.095.270511
CGACGTA251.8037796E-593.764146
GTCGTGT304.9549817E-793.1906213
GATCAGA305.139609E-792.6241151
GTTGGGA252.2806167E-589.4629918
GGTAGAG252.3511246E-588.919145
GTCGACG208.956279E-487.62344
AGGCGCC209.071051E-487.3439543
ATCTCTG209.541152E-486.24528535
GAGTGTA209.782907E-485.70624527
TAAGAGT209.782907E-485.70624524
Produced by FastQC (version 0.11.5)