FastQCFastQC Report
Tue 25 Oct 2016
6_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15302764
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGG32050.033.430378109
CTAGGCT34150.031.16489111
ACTAGGC36550.029.48889110
TGACTAG38550.028.66683108
GCTAGGT9300.024.936321113
CTAGAAC43550.024.51459172
CAGCTAG43900.024.38455669
TAATCTA44300.023.060658119
GTGACTA49650.022.660566107
CTAGGTT10900.022.04853114
CTAATCT46850.021.960114118
TAGGTTA11250.020.272652115
CTATAAC50700.020.1037277
CAACCTA50600.019.95004899
TAGCTAA19550.019.87789343
GACCTAA31500.019.73966698
GCTAGAA56700.019.32604671
ACCGCTA53000.019.038212115
CCAGCTA60050.018.8833468
AGACCTA32600.018.79699397
Produced by FastQC (version 0.11.5)