FastQCFastQC Report
Tue 25 Oct 2016
6_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6911
Sequences flagged as poor quality0
Sequence length3-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTAACATGAATCATTTCTCGATCCCATAGTTTTTCCTGTGAAATA110.15916654608594993No Hit
CTGCGCAGGATGTCCACTAATTCCGCCTGTGTCTGCAGGGCACCCAACTG80.11575748806250905No Hit
CTTGGAAACAAGTGGGAATAGAACCTGGAGACTCACACGTCCTTCTTTAT70.10128780205469541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTCC100.009136179132.12396117
TACGGCT100.009365626131.04097115
CGTACGG100.009365626131.04097113
TGGGATG100.009365626131.04097112
ACGGCTC100.009365626131.04097116
GTACGGC100.009365626131.04097114
TCTCGTT100.009598866129.9756110
ATTGATT100.009835932128.92741107
AGAGAAC100.009835932128.92741107
TGCCTCC350.0083106849.649079
Produced by FastQC (version 0.11.5)