FastQCFastQC Report
Tue 25 Oct 2016
6_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15302764
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT200110.1307672261037287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT276950.058.7000241
ACAGTTT395750.041.489622
CAGTTTC463400.035.3706553
GTTTCTG962650.034.341191
TTCTGTA1026100.031.8686393
TCTGTAC1033000.031.6838154
TGTACTA1093600.029.8194836
TTTCTGT1125100.029.28552
CTGTACT1117650.029.243595
TACTATA1122050.029.0849118
GTACTAT1137700.028.6941667
ACTATAT1168500.027.9353359
CTATATT1208000.027.11924710
AGTTTCT617300.026.721294
TATATTG1296850.025.24916311
ATACAGT108150.023.3059756
ATATTGG472750.022.33715212
GATACAG116600.022.1623955
TTGATAC130700.020.1698193
ATAGTAC28500.019.9244236
Produced by FastQC (version 0.11.5)